There's a strong gay subtext: drug dealer Ralph (Dave O'Brien) sees high schooler Jimmy (Warren McCollum), murmurs "Nice!", and practically licks his lips in anticipation. Wrangling an introduction, he says "Nice to meet youuuuuu!" with a lascivious leer, then invites Jimmy to the soda shop, where he will try to get him hooked on the psychosis-inducing weed in a parallel to how gay men were accused of recruiting boys.
The film version, Reefer Madness: The Movie Musical (2005), star Christian Campbell as Jimmy, Steven Weber as Jack, and Robert Torti as Jesus. It adds some characters, such as John Mann as Satan, ups the gay subtext by removing all pronouns from the seduction scenes, and adds an explicit connection between the anti-marijuana crusade of the 1930s with bigoted attacks on gays and lesbians:
We'll start on Darwin and Sigmund Freud
And sex depicted on celluloid
And communists and queens!
When danger's near, exploit their fear
The end will justify the means!